Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to  both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

Hersteller:
Humana Press in Springer Science + Business Media
juergen.hartmann@springer.com
Heidelberger Platz 3
DE 14197 Berlin

1.  Guide to the Book Plant Kinases             Nico Dissmeyer and Arp Schnittger   2. The Age of Protein Kinases             Nico Dissmeyer and Arp Schnittger   3. Expression and Purification of Active Protein Kinases from Wheat Germ Extracts             Boglarka Sonkoly, Viola Bardoczy, and Tamas Meszaros   4. Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System             Hirofumi Harashima and  Masami Sekine   5. Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants             Robert Doczi, Elizabeth Hatzimasoura, and Laszlo Bögre   6. Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks             Nico Dissmeyer and Arp Schnittger             7. Bacterial Assay to Study Plant Sensor Histidine Kinases             LukaÅ¡ Spíchal   8.  Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase             Julija Umbrasaite, Alois Schweighofer and Irute Meskiene   9. Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen             Shutian Li and Vernonica E. Franklin-Tong   10. Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules             Dor Salomon, Chao Zhang, Kevan M. Shokat, and Guido Sessa   11. Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization             Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser   12. Quantifying Degradation Rates of Trans-Membrane Receptor Kinases             Niko Geldner   13. Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta             Mark A.C.J. Kwaaitaal, Marieke Schor, Mark A. Hink, Antonie J.W.G. Visser, and Sacco C. de Vries   14. Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo             Stefan Pusch, Nico Dissmeyer, and Arp Schnittger   15. Chemical Genetic Analysis of Protein Kinase Function in Plants             Mike Böhmer, Michael Bölker, and Tina Romeis   16. Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes             Thomas Colby, Horst Röhrig, Anne Harzen, and Jürgen Schmidt   17. Phosphoproteomics Using iTRAQ             Alexandra M.E. Jones and Thomas S. Nühse