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Claudins

Methods and Protocols, Methods in Molecular Biology 762

Erschienen am 01.07.2011, 1. Auflage 2011
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Bibliografische Daten
ISBN/EAN: 9781617791840
Sprache: Englisch
Umfang: xiv, 447 S.
Einband: gebundenes Buch

Beschreibung

Inhaltsangabe1. Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling            Christina M. Van Itallie and James M. Anderson 2. Biochemical Analysis of Claudin Binding Compatibility            Christina Ward and Michael Koval 3. Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines            Alan S.L. Yu 4. The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Status            Makoto Osanai 5. Interactions between Clostridium perfringens Enterotoxin and Claudins            Susan L. Robertson and Bruce A. McClane 6. Biophysical Methods to Probe Claudin Mediated Adhesion at the Cellular and Molecular Level            Sri Ram Krishna Vedula, Tong Seng Lim, Walter Hunziker, and Chwee Teck Lim 7. Detection of Tight Junction Barrier Function In Vivo by Biotin            Lei Ding, Yuguo Zhang, Rodney Tatum, and Yan-Hua Chen 8. The Co-Culture Method to Examine Interactions between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells            Tetsuichiro Inai 9. Claudin 4: Functional Studies Beyond the Tight Junction            Holly A. Eckelhoefer, Thejani E. Rajapaksa, Jing Wang, Mary Stover-Hamer, Nancy C. Appleby, Jun Ling, and David D. Lo 10. Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin 16            P. Jaya Kausalya and Walter Hunziker 11. Claudin Family Proteins in C. elegans            Jeffrey S.Simske and Jeff Hardin 12. In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka            Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki 13. Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium            Phuong Bui and Scott P. Kelly 14. Manipulating Claudin Expression in Avian Embryos            Michelle M. Collins and Aimee K. Ryan 15. Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues            Lorenza González-Mariscal, Erika Garay, and Miguel Quirós 16. Expression and Function of Claudins in Hepatocytes            Takashi Kojima and Norimasa Sawada 17. Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture            Junko Fujita-Yoshigaki 18. Development of Biological Tools to Study Claudins in the Male Reproductive Tract            Daniel G. Cyr, Évemie Dubé, Julie Dufresne, and Mary Gregory 19. Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo            Julian A. Guttman 20. Laboratory Methods in the Study of Endometrial Claudin-4            Paulo Serafini, André Monteiro Rocha, Gary Daniel Smith, Eduardo Leme Alves da Motta, and Edmund Chada Baracat 21. Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction            Yu Sun, Richard D. Minshall, and Guochang Hu 22. Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells            Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic 23. Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier            He

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Inhalt

1. Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling             Christina M. Van Itallie and James M. Anderson   2. Biochemical Analysis of Claudin Binding Compatibility             Christina Ward and Michael Koval   3. Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines             Alan S.L. Yu   4. The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Status             Makoto Osanai   5. Interactions between Clostridium perfringens Enterotoxin and Claudins             Susan L. Robertson and Bruce A. McClane   6. Biophysical Methods to Probe Claudin Mediated Adhesion at the Cellular and Molecular Level             Sri Ram Krishna Vedula, Tong Seng Lim, Walter Hunziker, and Chwee Teck Lim   7. Detection of Tight Junction Barrier Function In Vivo by Biotin             Lei Ding, Yuguo Zhang, Rodney Tatum, and Yan-Hua Chen   8. The Co-Culture Method to Examine Interactions between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells             Tetsuichiro Inai   9. Claudin 4: Functional Studies Beyond the Tight Junction             Holly A. Eckelhoefer, Thejani E. Rajapaksa, Jing Wang, Mary Stover-Hamer, Nancy C. Appleby, Jun Ling, and David D. Lo   10. Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin 16             P. Jaya Kausalya and Walter Hunziker   11. Claudin Family Proteins in C. elegans             Jeffrey S.Simske and Jeff Hardin   12. In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka             Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki   13. Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium             Phuong Bui and Scott P. Kelly   14. Manipulating Claudin Expression in Avian Embryos             Michelle M. Collins and Aimee K. Ryan   15. Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues             Lorenza Gonzalez-Mariscal, Erika Garay, and Miguel Quiros   16. Expression and Function of Claudins in Hepatocytes             Takashi Kojima and Norimasa Sawada   17. Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture             Junko Fujita-Yoshigaki   18. Development of Biological Tools to Study Claudins in the Male Reproductive Tract             Daniel G. Cyr, Evemie Dube, Julie Dufresne, and Mary Gregory   19. Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo             Julian A. Guttman   20. Laboratory Methods in the Study of Endometrial Claudin-4             Paulo Serafini, Andre Monteiro Rocha, Gary Daniel Smith, Eduardo Leme Alves da Motta, and Edmund Chada Baracat   21. Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction             Yu Sun, Richard D. Minshall, and Guochang Hu   22. Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells             Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic   23. Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier             Heping Xu and Janet Liversidge   24. MMP-Mediated Disruption of Claudin-5 in the Blood-Brain Barrier of Rat Brain after Cerebral Ischemia             Yi Yang and Gary A. Rosenberg   25. Claudin-5 Expression in In Vitro Models of the Blood-Brain Barrier             Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I.  Teichberg   26. HIV-1-Induced Alterations of Claudin-5 Expression at the Blood Brain Barrier Level             Ibolya E. Andras and Michal Toborek   27. Enhanced Immunohistochemica ...

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